Introduction

This course will help you gain a sound working knowledge of basic, high-performance liquid chromatographic principles and develop a greater understanding of theoretical and practical relationships.

Build the expertise you need to choose optimized column parameters, detector selection, and separation mechanism for the examination of complex mixtures, and improve your on-the-job performance.

  • HPLC theory and best practices
  • Column optimization
  • Detector selection
  • Improve separation of complex mixtures

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High Performance Liquid Chromatography Basics Module 1

Module 1 – Introduction

  • Introduction to HPLC
  • Separation Mechanisms
  • Components of HPLC Instrumentation
  • Commercial HPLC Instruments


High Performance Liquid Chromatography Basics Module 2

Module 2 – Theory of HPLC

  • Selection of Separation Mechanism
  • Features of HPLC Chromatograms
  • Resolution of HPLC Columns
  • Resolution vs. Selectivity


High Performance Liquid Chromatography Basics Module 3

Module 3 – Instrumentation

  • Pumps
  • Columns
  • Detectors
  • Ultraviolet
  • Fluorescence
  • Mass Spectrometry
  • Refractive Index
  • Evaporative Light Scattering


High Performance Liquid Chromatography Basics Module 4

Module 4 – Separation Mechanisms

  • Adsorption
  • Partition
  • Normal Phase
  • Reverse Phase
  • Anionic and Cationic Ion Exchange
  • Steric Exclusion
  • Affinity


High Performance Liquid Chromatography Basics Module 5

Module 5 – Qualitative Analysis

  • Retention Time
  • Retention Indices
  • Ultraviolet Spectroscopy
  • Infrared Spectroscopy
  • Mass Spectroscopy


High Performance Liquid Chromatography Basics Module 6

Module 6 – Quantitative Analysis

  • Integration Methods
  • Area Percent
  • Use of Response Factors
  • Internal Standard
  • External Calibration
  • Method of Additions


High Performance Liquid Chromatography Basics Module 7

Module 7 – Alternate and Optimization Techniques

  • Enhanced UV Detection
  • Capillary Zone Electrophoresis
  • Octanol / Water Partition Coefficients
  • Gradient Optimization
  • Methods Development Criteria
  • Computer Based Optimization